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Can I read Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome on EtoBox?
Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome by Paul B. Rainey; Mark J. Bailey is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome about?
__Pseudomonas fluorescens__ is a saprophytic bacterium commonly isolated from soil, water, and the surfaces and tissues of plants and animals. The species has important applications in biotechnology because it can enhance plant growth and protect crops against disease. A complete physical map of the 6.63 Mbp __P. fluorescens__ SBW25 chromosome was constructed using data obtained from combinations of one‐ and two‐dimensional electrophoresis of completely or partially digested chromosomal DNA with end labelling. In total, 139 restriction sites (15 __Pac__I, 53 __Spe__I, 71 __Xba__I) were placed on the physical map and complete maps of the circular chromosome were obtained for both __Pac__I and __Spe__I; only __Xba__I fragments linking __Spe__I fragments were positioned. The average resolution of restriction sites was 48 kbp. A genetic map was derived from the physical map by Southern hybridization and 31 genes were positioned including __oriC__, rDNA operons (__rnnA–E__), __recA____gacA__ and __pyvD__
Who reads Physical and genetic map of the Pseudomonas fluorescens SBW25 chromosome?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Paul B. Rainey; Mark J. Bailey
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 1996
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)