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Quantification of artemisinin in Artemisia annua extracts by 1H-NMR by Paula C. Castilho; Sandra C. Gouveia; Ana I. Rodrigues is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Artemisinin is a polycyclic sesquiterpene lactone that is highly effective against multidrug‐resistant strains of __Plasmodium falciparum__, the etiological agent of the most severe form of malaria. Determination of artemisinin in the source plant, __Artemisia annua__, is a challenging problem since the compound is present in very low concentrations, is thermolabile and unstable, and lacks chromophoric or fluorophoric groups. The ain of this study was to develop a simple protocol for the quantification of artemisinin in a plant extract using an ^1^H‐NMR method. Samples were prepared by extraction of leaf material with acetone, treatment with activated charcoal to remove chlorophylls and removal of solvent. ^1^H‐NMR spectra were measured on samples dissolved in deuterochloroform with __tert__‐butanol as internal standard. Quantification was carried out using the $\d{$\delta$}$ 5.864 signal of artemisinin and the __δ__ 1.276 signal of __tert__‐butanol. The method was optimised and fully validated against a reference standard of artemisinin. The results were compared with those obtained from the same samples quantified using an HPLC‐refractive index (RI) method. The ^1^H‐N

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Author

Paula C. Castilho; Sandra C. Gouveia; Ana I. Rodrigues

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)