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Can I read Deciphering the Temporal Heterogeneity of Cancer-associated Fibroblast Subpopulations in Breast Cancer on EtoBox?
Deciphering the Temporal Heterogeneity of Cancer-associated Fibroblast Subpopulations in Breast Cancer by Freja Albjerg Venning; Kamilla Westarp Zornhagen; Lena Wullkopf; Jonas Sjölund; Carmen Rodriguez-Cupello; Pontus Kjellman; Mikkel Morsing; Morteza Chalabi Hajkarim; Kyoung Jae Won; Janine Terra Erler; Chris Denis Madsen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Deciphering the Temporal Heterogeneity of Cancer-associated Fibroblast Subpopulations in Breast Cancer about?
## Background Cancer-associated fibroblasts (CAFs) comprise a heterogeneous population of stromal cells within the tumour microenvironment. CAFs exhibit both tumour-promoting and tumour-suppressing functions, making them exciting targets for improving cancer treatments. Careful isolation, identification, and characterisation of CAF heterogeneity is thus necessary for ex vivo validation and future implementation of CAF-targeted strategies in cancer. ## Methods Murine 4T1 (metastatic) and 4T07 (poorly/non-metastatic) orthotopic triple negative breast cancer tumours were collected after 7, 14, or 21 days. The tumours were analysed via flow cytometry for the simultaneous expression of six CAF markers: alpha smooth muscle actin (αSMA), fibroblast activation protein alpha (FAPα), platelet derived growth factor receptor alpha and beta (PDGFRα and PDGFRβ), CD26/DPP4 and podoplanin (PDPN). All non-CAFs were excluded from the analysis using a lineage marker cocktail (CD24, CD31, CD45, CD49f, EpCAM, LYVE-1, and TER-119). In total 128 murine tumours and 12 healthy mammary fat pads were analysed. ## Results We have developed a multicolour flow cytometry strategy based on exclusion of non-CAFs a
Who reads Deciphering the Temporal Heterogeneity of Cancer-associated Fibroblast Subpopulations in Breast Cancer?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Freja Albjerg Venning; Kamilla Westarp Zornhagen; Lena Wullkopf; Jonas Sjölund; Carmen Rodriguez-Cupello; Pontus Kjellman; Mikkel Morsing; Morteza Chalabi Hajkarim; Kyoung Jae Won; Janine Terra Erler; Chris Denis Madsen
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)