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Enzyme activity and population genetic structure analysis in wheat associated with resistance to Bipolaris sorokiniana-comm ... 1 1 Introduction 1 2 Results 2 2.1 Disease severity evaluation on different wheat genotypes 2 2.2 Evaluation of defense-related enzyme activities 2 2.3 SSR analysis 3 3 Discussion 5 4 Conclusion 7 5 Experimental 7 5.1 Triticum aestivum plant material sources and growth conditions 7 5.2 Inoculation of Triticum aestivum with CRR pathogen, B. sorokiniana for defense-related enzyme activities 7 5.3 Evaluation of defense-related enzyme activities 8 5.3.1 Total protein content 8 5.3.2 Superoxide dismutase activity 8 5.3.3 Peroxidase activity 8 5.3.4 Polyphenol oxidase activity 8 5.3.5 Catalase activity 8 5.3.6 Phenylalanine ammonia-lyase activity 8 5.3.7 1,3-β-glucanase activity 8 5.3.8 Chitinase activity 8 5.3.9 Total phenol content 8 5.4 SSR analysis 8 5.4.1 DNA extraction 8 5.4.2 PCR conditions 9 5.4.3 Genetic analyses 9 6 Statistical analysis 9 Ethical approval 9 Funding 9 Author contributions 9 Declaration of competing interest 9 Acknowledgment 9 Appendix A Supplementary data 9 References 9

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nonfiction