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Malaria infection by sporozoite challenge induces high functional antibody titres against blood stage antigens after a DNA prime, poxvirus boost vaccination strategy in Rhesus macaques by Muzamil Mahdi Abdel Hamid; Edmond J Remarque; Ibrahim M El Hassan; Ayman A Hussain; David L Narum; Alan W Thomas; Clemens HM Kocken; Walter R Weiss; Bart W Faber is a Medicine article available to read on EtoBox.

What is Malaria infection by sporozoite challenge induces high functional antibody titres against blood stage antigens after a DNA prime, poxvirus boost vaccination strategy in Rhesus macaques about?

## Background A DNA prime, poxvirus (COPAK) boost vaccination regime with four antigens, i.e. a combination of two __Plasmodium knowlesi__ sporozoite (__csp/ssp2__) and two blood stage (__ama1/msp1__~__42__~) genes, leads to self-limited parasitaemia in 60% of rhesus monkeys and survival from an otherwise lethal infection with __P. knowlesi__. In the present study, the role of the blood stage antigens in protection was studied in depth, focusing on antibody formation against the blood stage antigens and the functionality thereof. ## Methods Rhesus macaques were immunized with the four-component vaccine and subsequently challenged i.v. with 100 __P. knowlesi__ sporozoites. During immunization and challenge, antibody titres against the two blood stage antigens were determined, as well as the __in vitro__ growth inhibition capacity of those antibodies. Antigen reversal experiments were performed to determine the relative contribution of antibodies against each of the two blood stage antigens to the inhibition. ## Results After vaccination, PkAMA1 and PkMSP1~19~ antibody titres in vaccinated animals were low, which was reflected in low levels of inhibition by these antibodies as determ

Who reads Malaria infection by sporozoite challenge induces high functional antibody titres against blood stage antigens after a DNA prime, poxvirus boost vaccination strategy in Rhesus macaques?

Author

Muzamil Mahdi Abdel Hamid; Edmond J Remarque; Ibrahim M El Hassan; Ayman A Hussain; David L Narum; Alan W Thomas; Clemens HM Kocken; Walter R Weiss; Bart W Faber

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Research Square (ISSN 1475-2875)

Published

2011

Language

EN

Field

Medicine (Health Sciences)