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Synthetic Mannosides Act as Acceptors for Mycobacterial α1-6 Mannosyltransferase by Jillian R Brown; Robert A Field; Adam Barker; Mark Guy; Ravinder Grewal; Kay-Hooi Khoo; Patrick J Brennan; Gurdyal S Besra; Delphi Chatterjee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Synthetic Mannosides Act as Acceptors for Mycobacterial α1-6 Mannosyltransferase about?
AbstractÐA series of synthetic mannosides was screened in a cell-free system for their ability to act as acceptor substrates for mycobacterial mannosyltransferases. Evaluation of these compounds demonstrated the incorporation of [ 14 C]Man from GDP-[ 14 C]Man into a radiolabeled organic-soluble fraction and analysis by thin layer chromatography and autoradiography revealed the formation of two radiolabeled products. Each synthetic acceptor was capable of accepting one or two mannose residues, resulting in a major and a minor mannosylated product. Both products from each acceptor were isolated and their mass was con®rmed by fast-atom bombardment±mass spectrometry (FABMS). Characterization of each mannosylated product by exo-glycosidase digestion, acetolysis and linkage analysis by gas chromatography±mass spectrometry of partially per-O-methylated alditols, revealed only a1-6-linked products. In addition, the antibiotic amphomycin selectively inhibited the formation of mannosylated products suggesting polyprenolmonophosphate-mannose (C 35/50 -P-Man) was the immediate mannose donor in all mannosylation reactions observed. The ability of synthetic disaccharides to act as acceptor subst
Who reads Synthetic Mannosides Act as Acceptors for Mycobacterial α1-6 Mannosyltransferase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jillian R Brown; Robert A Field; Adam Barker; Mark Guy; Ravinder Grewal; Kay-Hooi Khoo; Patrick J Brennan; Gurdyal S Besra; Delphi Chatterjee
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0968-0896)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)