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Somatic embryogenesis in mature zygotic embryos of Picea likiangensis by Shaoyu Chen; Shanna Chen; Fang Chen; Tao Wu; Yinbin Wang; Shanjun Yi is a Agricultural and Biological Sciences article available to read on EtoBox.

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Somatic embryogenesis (SE) was successfully induced from mature zygotic embryos of seven families of Picea likiangensis (Franch.) Pritz after 20 weeks culture on initiation medium. Three basal media (one-half strength LM medium, one-half strength LP medium and improved LP medium) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA) were tested but only one-half strength LM medium supplemented with 2,4-D and 6-BA was successful for the embryogenic cultures (EC) initiation. The initiation frequencies of EC varied greatly from different families when culturing on the same initiation medium. The highest frequency (41.3%) was induced from one of the families on one-half strength LM medium supplemented with 3 mg L -1 2,4-D and 1.5 mg L -1 6-BA and 16.83% on average for seven families. EC were subcultured and proliferated on the same medium as the initiation one every 10 days. 3 lines of EC induced from the same family were applied in maturation experiment. Cotyledonary somatic embryos were observed after EC were transferred to maturation media of one-half strength LM medium containing 20-80 mg L -1 abscisic acid and 7.5% polyethylene glycol (

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Author

Shaoyu Chen; Shanna Chen; Fang Chen; Tao Wu; Yinbin Wang; Shanjun Yi

Publisher

Springer Science and Business Media LLC

Published

2010

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)