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Can I read CN‐3 increases TMZ sensitivity and induces ROS‐dependent apoptosis and autophagy in TMZ‐resistance glioblastoma on EtoBox?
CN‐3 increases TMZ sensitivity and induces ROS‐dependent apoptosis and autophagy in TMZ‐resistance glioblastoma by Yu‐Ye Xue; Yun‐Yang Lu; Guang‐Qiang Sun; Fei Fang; Yu‐Qiang Ji; Hai‐Feng Tang; Peng‐Cheng Qiu; Guang Cheng is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is CN‐3 increases TMZ sensitivity and induces ROS‐dependent apoptosis and autophagy in TMZ‐resistance glioblastoma about?
## Abstract Many glioma patients develop resistance to temozolomide (TMZ) treatment, resulting in reduced efficacy and survival rates. TMZ‐resistant cell lines SHG44R and U87R, which highly express O~6~‐methylguanine DNA methyltransferase (MGMT) and P‐gp, were established. CN‐3, a new asterosaponin, showed cytotoxic effects on TMZ‐resistant cells in a dose‐ and time‐dependent manner via reactive oxygen species (ROS)‐mediated apoptosis and autophagy. Transmission electron microscopy and monodansylcadaverine (MDC) staining showed turgidity of the mitochondria and autophagosomes in CN‐3‐treated SHG44R and U87R cells. The autophagy inhibitor 3‐methyladenine was used to confirm the important role of autophagy in CN‐3 cytotoxicity in TMZ‐resistant cells. The ROS scavenger N‐acetyl‐ l‐cysteine (NAC) attenuated the levels of ROS induced by CN‐3 and, therefore, rescued the CN‐3 cytotoxic effect on the viability of SHG44R and U87R cells by Cell Counting Kit‐8 assays and JuLI‐Stage videos. MDC staining also confirmed that NAC rescued an autophagosome increase in CN‐3‐treated SHG44R and U87R cells. Western blotting revealed that CN‐3 increased Bax, cleaved‐caspase 3, cytochrome C, PARP‐1, LC3‐
Who reads CN‐3 increases TMZ sensitivity and induces ROS‐dependent apoptosis and autophagy in TMZ‐resistance glioblastoma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yu‐Ye Xue; Yun‐Yang Lu; Guang‐Qiang Sun; Fei Fang; Yu‐Qiang Ji; Hai‐Feng Tang; Peng‐Cheng Qiu; Guang Cheng
- Publisher
- Wiley
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)