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Irreversible Adsorption of Human Serum Albumin to Hydrogel Contact Lenses: a Study Using Electron Spin Resonance Spectroscopy by Qian Garrett; Hans J. Griesser; Bruce K. Milthorpe; R.Wayne Garrett is a Engineering article available to read on EtoBox.

What is Irreversible Adsorption of Human Serum Albumin to Hydrogel Contact Lenses: a Study Using Electron Spin Resonance Spectroscopy about?

Human serum albumin (HSA) was speci"cally spin labelled with 4-maleimido-tempo (MSL) at its cysteine 34 residue (HSA-MSL). The irreversible adsorption of HSA-MSL to hydrogel contact lenses (eta"lcon A, te"lcon and vi"lcon A) was investigated using electron spin resonance (ESR) spectroscopy. Changes in ESR spectral characteristics of adsorbed HSA-MSL as compared to HSA-MSL in solution displayed an additional immobilisation of the spin label due to the adsorption. This immobilisation of MSL corresponds to a large conformational alteration of the HSA-MSL near the modi"ed Cys 34 residue. For both eta"lcon A and te"lcon, the rate of irreversible adsorption was relatively slow compared with that of vi"lcon A where the maximum state of immobilisation and hence conformational change occurred within the "rst hour of adsorption. Furthermore, te"lcon produced markedly di!erent ESR spectra where a strong conformational change to a less mobile protein was apparent. This supported a model where the direct irreversible adsorption of HSA from solution dominated on te"lcon as opposed to conversion of the adsorbed protein from the reversible to the irreversible state on both eta"lcon A and vi"lcon A

Who reads Irreversible Adsorption of Human Serum Albumin to Hydrogel Contact Lenses: a Study Using Electron Spin Resonance Spectroscopy?

Author

Qian Garrett; Hans J. Griesser; Bruce K. Milthorpe; R.Wayne Garrett

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

1999

Language

EN

Field

Engineering (Physical Sciences)