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Enzymatic supported synthesis of lacto-N-neotetraose using dendrimeric polyethylene glycol by Laetitia Renaudie; Richard Daniellou; Claudine Augé; Christine Le Narvor is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The lacto-N-neotetraose tetrasaccharide was synthesized on a new dendrimeric support, based on polyethylene glycol. Starting from 1-thio-beta-D-lactose, the trisaccharide (2-acetamido-2-deoxy-beta-D-glucopyranosyl)-(1-->3)-O-beta-D-galactopyranosyl-(1-->4)-1-thio-beta-D-glucopyranose was obtained using Neisseria meningitidis beta-(1-->3)-N-acetylglucosaminyltransferase according to a soluble synthesis approach, bound on the support and galactosylated using the milk beta-(1-->4)-galactosyl transferase to give after cleavage the tetrasaccharide lacto-N-neotetraose.

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Author

Laetitia Renaudie; Richard Daniellou; Claudine Augé; Christine Le Narvor

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)