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Phytotoxic effects on chloroplast and UHPLC-HRMS based untargeted metabolomic responses in Allium tuberosum Rottler ex Spre ... 1 1 Introduction 1 2 Material and methods 2 2.1 Chemicals 2 2.2 Experimental design and stress application 2 2.3 Chlorophyll fluorescence measurement 2 2.4 Transmission electron microscopy (TEM) 2 2.5 Quantification of antibiotics 2 2.6 Metabolic analysis 2 2.7 Metabolic data analysis 3 2.8 Accumulation and translocation factor 3 2.9 Statistical analysis 3 3 Results 3 3.1 Growth variables 3 3.2 Chlorophyll fluorescence 3 3.3 Transmission electron microscopy (TEM) 3 3.4 Antibiotic concentration 3 3.5 Accumulation coefficient and translocation factor 3 3.6 Metabolic analysis 7 3.6.1 Evaluation of stress responses through heat map 7 3.6.2 Multivariate analysis of identified metabolites 7 4 Discussion 7 4.1 Growth variables 7 4.2 Chlorophyll fluorescence variables 8 4.3 Transmission electron microscopy (TEM) 9 4.4 Antibiotics concentration 10 4.5 Metabolic analysis 10 5 Conclusion 12 CRediT authorship contribution statement 16 Declaration of Competing Interest 16 Acknowledgement 16 Appendix A Supporting information 16 References 16

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UND

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nonfiction