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Functional analysis of plastid DNA replication origins in tobacco by targeted inactivation by Stefan K. Mühlbauer; Andreas Lössl; Lilia Tzekova; Zhurong Zou; Hans-Ulrich Koop is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Functional analysis of plastid DNA replication origins in tobacco by targeted inactivation about?

## Summary Sequences described as chloroplast DNA replication origins were analysed __in vivo__ by creating deletion and insertion mutants via plastid transformation in tobacco. Deletion of the described __oriA__ sequence, which is located within the intron of the __trnI__ gene, resulted in heteroplastomic transformants, when the selection marker was inserted within the intron. Removal of the complete intron sequence together with the __oriA__ sequence, however, yielded homoplastomic transformants of normal phenotype, in which wild‐type signals were no longer detectable through Southern analysis, thus bringing the role of the described __oriA__ sequence for plastome replication into question. Similarly, deletion of sequence elements upstream of __trnI__, which have a possible ori function in __Oenothera__, did not show any effect in tobacco. The two copies of __oriB__, which are located at the very end of the plastome Inverted Repeats, were targeted with two different transformation vectors in a cotransformation approach. While in initial transformants integration of the selection marker could be detected at both sites, the transgene was found exclusively at one site or the other a

Who reads Functional analysis of plastid DNA replication origins in tobacco by targeted inactivation?

Author

Stefan K. Mühlbauer; Andreas Lössl; Lilia Tzekova; Zhurong Zou; Hans-Ulrich Koop

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2002

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)