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High-performance Liquid Chromatographic Determination of Etilefrine in Human Plasma Using Combined Solid-phase and Organic Solvent Extraction and Electrochemical Detection by Kenji Kojima; Mika Yamanaka; Yutaka Nakanishi; Satoshi Arakawa is a Chemistry article available to read on EtoBox.

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Etilefrine hydrochloride, %ethylamino-l-(3-hydroxyphenyl)ethanol hydrochloride (Fig. 1)) is an amine with sympathomimetic activity that has been used for many years in the treatment of orthostatic disorders [ 11. It undergoes considerable first-pass metabolism passing through the gut wall and shows inter-individual variation in bioavailability [ 21. It is important, therefore, to monitor the plasma levels in clinical research. Gas chromatography with mass spectrometry [ 3 ] and liquid scintillation counting of the radioactively labelled drug [ 41 have been the principal methods used to determine etilefrine in plasma and urine for pharmacokinetic studies. The high-performance liquid chromatographic (HPLC) methods are accurate and selective, but the sensitivity for etilefrine is not sufficient to enable the use of UV detection. Recently HPLC coupled with electrochemical detection has been widely used for the determination of drugs in biological fluids, because of its enhanced selectivity and sensitivity [ 51. This paper describes a reversed-phase HPLC Fig. 1. Structures etilefrine hydrochloride

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Author

Kenji Kojima; Mika Yamanaka; Yutaka Nakanishi; Satoshi Arakawa

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1990

Language

EN

Field

Chemistry (Physical Sciences)