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Assessing Colorectal Cancer Mismatch Repair Status in the Modern Era: a Survey of Current Practices and Re-evaluation of the Role of Microsatellite Instability Testing by Erika Hissong; Elizabeth P. Crowe; Rhonda K. Yantiss; Yao-Tseng Chen is a Medicine article available to read on EtoBox.

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Results of DNA mismatch repair testing are used to detect Lynch syndrome and have prognostic and therapeutic implications among patients with sporadic colorectal carcinomas. Immunohistochemistry for mismatch repair proteins (MLH1, PMS2, MSH2, MSH6) and PCR for microsatellite instability are two established methods for assessing mismatch repair function. Older literature suggested a discordance rate of approximately 5% between these assays, leading some institutions to perform dual testing on all cases. Although universal mismatch repair testing is now recommended by multiple professional organizations, none provide guidelines regarding preferred assays. We surveyed 96 academic and nonacademic institutions to assess Lynch syndrome screening practices and evaluated discordance rates between immunohistochemistry and PCR among 809 colorectal cancers tested in our own institution. Our survey demonstrated no significant differences between academic and nonacademic practices with respect to testing strategies. Eighty six percent performed universal screening, and usually (76%) employed immunohistochemistry on initial biopsy samples. Only 20% employed PCR; these were mostly academic practi

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Author

Erika Hissong; Elizabeth P. Crowe; Rhonda K. Yantiss; Yao-Tseng Chen

Publisher

Elsevier BV

Published

2018

Language

EN

Field

Medicine (Health Sciences)