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What is Amino Acid Analysis via Ion Exchange about?

This document reviews ion exchange chromatography for amino acid analysis. It discusses how ion exchange chromatography works through reversible adsorption of charged amino acid molecules to an immobilized ion exchange matrix. It also summarizes different hydrolysis methods used to break peptide bonds and release amino acids from proteins, including acid and alkaline hydrolysis. Acid hydrolysis using 6N HCl is commonly used but can destroy some amino acids. The document concludes that ion exchange chromatog

Author

jkc college

Language

EN