Can I read Isotope Tag Method for Quantitative Analysis of Carbohydrates by Liquid Chromatography–mass Spectrometry on EtoBox?

Isotope Tag Method for Quantitative Analysis of Carbohydrates by Liquid Chromatography–mass Spectrometry by Jin Yuan; Noritaka Hashii; Nana Kawasaki; Satsuki Itoh; Toru Kawanishi; Takao Hayakawa is a Chemistry article available to read on EtoBox.

What is Isotope Tag Method for Quantitative Analysis of Carbohydrates by Liquid Chromatography–mass Spectrometry about?

We have previously demonstrated that liquid chromatography/mass spectrometry equipped with a graphitized carbon column (GCC-LC/MS) is useful for the structural analysis of carbohydrates in a glycoprotein. Here, we studied the monosaccharide composition analysis and quantitative oligosaccharide profiling by GCC-LC/MS. Monosaccharides were labeled with 2-aminopyridine and then separated and monitored by GCC-LC/MS in the selective ion mode. The use of tetradeuterium-labeled pyridylamino (d4-PA) monosaccharides as internal standards, which were prepared by the tagging of standard monosaccharides with hexadeuterium-labeled 2-aminopyridine (d6-AP), afforded a good linearity and reproducibility in ESIMS analysis. This method was successfully applied to the monosaccharide composition analysis of model glycoproteins, fetuin, and erythropoietin. For quantitative oligosaccharide profiling, oligosaccharides released from an analyte and a standard glycoprotein were tagged with d0- and d6-AP, respectively, and an equal amount of d0- and d4-PA oligosaccharides were coinjected into GCC-LC/MS. In this procedure, the oligosaccharides that existed in either analyte or a standard glycoprotein appeared

Who reads Isotope Tag Method for Quantitative Analysis of Carbohydrates by Liquid Chromatography–mass Spectrometry?

Author

Jin Yuan; Noritaka Hashii; Nana Kawasaki; Satsuki Itoh; Toru Kawanishi; Takao Hayakawa

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)