Opening book details…
Can I read Design, Synthesis and Characterisation of Affinity Ligands for Glycoproteins on EtoBox?
Design, Synthesis and Characterisation of Affinity Ligands for Glycoproteins by Uma D. Palanisamy; Abid Hussain; Sayeedha Iqbal; Kenny Sproule; Christopher R. Lowe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Design, Synthesis and Characterisation of Affinity Ligands for Glycoproteins about?
The concepts of rational design and solid phase combinatorial chemistry were used to develop affinity adsorbents for glycoproteins. A detailed assessment of protein-carbohydrate interactions was used to identify key residues that determine monosaccharide specificity, which were subsequently exploited as the basis for the synthesis of a library of glycoprotein binding ligands. The ligands were synthesised using solid phase combinatorial chemistry and were assessed for their sugar-binding ability with the glycoenzymes, glucose oxidase and RNase B. Partial and completely deglycosylated enzymes were used as controls. The triazine-based ligand, histamine/tryptamine (8/10) was identified as a putative glycoprotein binding ligand, since it displayed particular affinity for glucose oxidase and other mannosylated glycoproteins. Experiments with deglycosylated control proteins, specific eluants and retardation in the presence of competing sugars strongly suggest that the ligand binds the carbohydrate moiety of glucose oxidase rather than the protein itself.
Who reads Design, Synthesis and Characterisation of Affinity Ligands for Glycoproteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Uma D. Palanisamy; Abid Hussain; Sayeedha Iqbal; Kenny Sproule; Christopher R. Lowe
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3499)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)