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Amperometric biosensor based on Prussian Blue-modified screen-printed electrode for lipase activity and triacylglycerol determination by Ines Ben Rejeb; Fabiana Arduini; Aziz Amine; Mohamed Gargouri; Giuseppe Palleschi is a Chemistry article available to read on EtoBox.
What is Amperometric biosensor based on Prussian Blue-modified screen-printed electrode for lipase activity and triacylglycerol determination about?
Lipase activity against triacylglycerols has been measured using an amperometric enzyme biosensor based on glycerol dehydrogenase/NADH oxidase. A Prussian Blue modified screen-printed electrode was selected as substrate for the two immobilised-enzyme systems due to their higher operative stability reported in previous works. Various parameters such as cofactor (flavin mononucleotide FMN) concentration (1 mM), NAD + coenzyme concentration (2 mM), pH effect (phosphate buffer pH 6 to 8, Tris buffer pH 8-10) response time and storage stability were evaluated and optimised. The glycerol biosensor was then investigated for lipase activity. The system was challenged against an olive or sunflower oil real samples in order to detect fatty acids and the results were compared with those provided either by the manufacture or reference methods with good agreement.
Who reads Amperometric biosensor based on Prussian Blue-modified screen-printed electrode for lipase activity and triacylglycerol determination?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Ines Ben Rejeb; Fabiana Arduini; Aziz Amine; Mohamed Gargouri; Giuseppe Palleschi
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)