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Can I read Evaluation of Surface Plasmon Resonance (SPR) for Heparin Assay on EtoBox?
Evaluation of Surface Plasmon Resonance (SPR) for Heparin Assay by Katharina Gaus; Elizabeth A.H. Hall is a Chemistry article available to read on EtoBox.
What is Evaluation of Surface Plasmon Resonance (SPR) for Heparin Assay about?
Heparin adsorption onto polyethylene imine (PEI) films was investigated. The adsorption was followed with time-dependent surface plasmon resonance (SPR) for PEI cast on gold films, and the thickness of the adsorbed heparin was determined by fitting the angular reflection curves to Fresnel's equations. The average thickness of the PEI determined the heparin adsorption range and it was found that both heparin binding capacity and sensitivity increased with PEI thickness. At an appropriate thickness of 10 ± 2 nm, adsorption of heparin in the clinically applicable range of 0-2 U/ml caused a linear concentration dependent change in the SPR reflectivity at a fixed angle, with excellent sensitivity (0.05 U/ml). The results are compared to heparin adsorption on immobilized protamine and the surface coverage and probable heparin distribution discussed. Copyright 1997 Academic Press. Copyright 1997Academic Press
Who reads Evaluation of Surface Plasmon Resonance (SPR) for Heparin Assay?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Katharina Gaus; Elizabeth A.H. Hall
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0021-9797)
- Published
- 1997
- Language
- EN
- Field
- Chemistry (Physical Sciences)