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Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis by Nor Hassifi Shuib; Khozirah Shaari; Alfi Khatib; Maulidiani; Ralf Kneer; Seema Zareen; Salahudin Mohd. Raof; Nordin Hj. Lajis; Victor Neto is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis about?
The 'Ulam', a traditional Malay dish, are plants that can be eaten raw, as a form of local salad. The shoots and young leaves of Melicope ptelefolia are among the popular species, believed to be high in nutritional and medicinal values. The metabolomic fingerprinting analysis of the ethanolic extracts of leaves of M. ptelefolia was carried out using 1 H Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate data analysis in order to differentiate young and mature leaves and to evaluate the variation of their chemical composition. Principle component analysis (PCA) of the 1 H NMR spectra showed a clear discrimination between the young and mature leaves extracts by PC3 and PC4. The compounds responsible for the differentiation were identified by comparison of 1 H NMR chemical shifts and qualitative HPLC. The young leaves were found to be richer in fatty acids and the levels of the three marker compounds, p-Ogeranylcoumaric acid, 2,4,6-trihydroxy-3-geranylacetophenone and 2,4,6-trihydroxy-3-prenylacetophenone, were clearly higher. The mature leaves contain higher levels of sugars and glycosidic components.
Who reads Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysis?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Nor Hassifi Shuib; Khozirah Shaari; Alfi Khatib; Maulidiani; Ralf Kneer; Seema Zareen; Salahudin Mohd. Raof; Nordin Hj. Lajis; Victor Neto
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)
- Published
- 2011
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)