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Studying the effect of storage conditions on the metabolite content of red wine using HILIC LC–MS based metabolomics by Panagiotis Arapitsas; Anna Della Corte; Helen Gika; Luca Narduzzi; Fulvio Mattivi; Georgios Theodoridis is a Agricultural and Biological Sciences article available to read on EtoBox.

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The main aim of this work was to develop an untargeted normal phase LC-MS method, starting from a targeted method already validated for the analysis of 135 polar metabolites. Since the LC instrument and column were the same, most of the chromatographic conditions remained identical, while the adaptations focused on maintaining the ionic strength of the eluents constant. The sample preparation was simplified and the effectiveness of LC-MS for long batches was evaluated, in order to record the maximum number of metabolites with good chromatographic resolution and the best MS stability and accuracy. The method was applied to study the influence of storage conditions on wine composition. Slightly sub-optimum storage conditions had a major impact on the polar metabolite fingerprint of the red wines analysed and the markers revealed included phenolics, vitamins and metabolites indentified in wine for the first time (4-amino-heptanedioic acid and its ethyl ester).

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Author

Panagiotis Arapitsas; Anna Della Corte; Helen Gika; Luca Narduzzi; Fulvio Mattivi; Georgios Theodoridis

Published

2015

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)