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Synergistic Anticancer Activity of Resveratrol in Combination with Docetaxel in Prostate Carcinoma Cells by Sang-Han Lee; Yoon-Jin Lee is a Medicine article available to read on EtoBox.

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## BACKGROUND/OBJECTIVES The study was conducted to investigate the efficacy of the combination treatment of phytochemical resveratrol and the anticancer drug docetaxel (DTX) on prostate carcinoma LNCaP cells, including factors related to detailed cell death mechanisms. ## MATERIALS/METHODS Using 2-dimensional monolayer and 3-dimensional spheroid culture systems, we examined the effects of resveratrol and DTX on cell viability, reactive oxygen species (ROS) levels, mitochondrial membrane potential, apoptosis, and necroptosis by MTT, flow cytometry, and Western blotting. ## RESULTS At concentrations not toxic to normal human prostate epithelial cells, resveratrol effectively decreased the viability of LNCaP cells depending on concentration and time. The combination treatment of resveratrol and DTX exhibited synergistic inhibitory effects on cell growth, demonstrated by an increase in the sub-G~0~/G~1~ peak, Annexin V-phycoerythrin positive cell fraction, ROS, mitochondrial dysfunction, and DNA damage response as well as concurrent activation of apoptosis and necroptosis. Apoptosis and necroptosis were rescued by pretreatment with ROS scavenger N-acetylcysteine. ## CONCLUSIONS We rep

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Author

Sang-Han Lee; Yoon-Jin Lee

Publisher

The Korean Nutrition Society and The Korean Society of Community Nutrition

Published

2020

Language

EN

Field

Medicine (Health Sciences)