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Phenotypic Effects of Continuous or Discontinuous Treatment with Dexamethasone And/or Calcitriol on Osteoblasts Differentiated from Rat Bone Marrow Stromal Cells by Hassan Atmani; Christine Audrain; Louis Mercier; Daniel Chappard; Michel F. Basle is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Phenotypic Effects of Continuous or Discontinuous Treatment with Dexamethasone And/or Calcitriol on Osteoblasts Differentiated from Rat Bone Marrow Stromal Cells about?
## Abstract Osteoblasts are target cells for glucocorticoids and calcitriol, and their phenotype is greatly modified by these hormones. We investigated the effect of continuous or discontinuous hormonal exposure to osteoblasts derived from rat bone marrow stromal cells in long‐term subcultures. Stromal cells were grown in primoculture in presence of dexamethasone (dex), but in following subcultures, dex and/or calcitriol were added just after seeding or after a 7‐day hormone‐free period. Cell proliferation, alkaline phosphatase (ALP) histochemical staining, and enzymatic bioactivity measurement, osteocalcin (OC), ALP and bone sialoprotein (BSP) mRNA expression were used to study the differential effect on osteoblastic phenotype of various conditions of treatment by dex and calcitriol. In primoculture, the osteoblastic differentiation was confirmed by the formation of calcified nodules and by strong expression of ALP, OC, and BSP mRNAs. In subcultures, proliferation of stromal cells was stimulated by dex and inhibited by calcitriol and by both hormones. Cell proliferation was not modified by hormonal lack during 7 days. Continuous hormonal treatment by dex strongly enhanced OC and B
Who reads Phenotypic Effects of Continuous or Discontinuous Treatment with Dexamethasone And/or Calcitriol on Osteoblasts Differentiated from Rat Bone Marrow Stromal Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hassan Atmani; Christine Audrain; Louis Mercier; Daniel Chappard; Michel F. Basle
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)