Can I read Redox-sensitive Proteome and Antioxidant Strategies in Wheat Seed Dormancy Control on EtoBox?

Redox-sensitive Proteome and Antioxidant Strategies in Wheat Seed Dormancy Control by Natalia V. Bykova; Brenda Hoehn; Christof Rampitsch; Travis Banks; Jo-Ann Stebbing; Tao Fan; Ron Knox is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Redox-sensitive Proteome and Antioxidant Strategies in Wheat Seed Dormancy Control about?

## Abstract Oxidative signalling by ROS has been demonstrated to play a role in seed dormancy alleviation, but the detailed molecular mechanisms underlying this process remain largely unknown. Here, we show dynamic differences in redox‐sensitive proteome upon wheat seed dormancy release. Using thiol‐specific fluorescent labelling, solubility‐based protein fractionation, 2‐D IEF PAGE, and MS analysis in conjunction with wheat EST sequence libraries, proteins with reversible oxidoreductive changes were characterized. Altogether, 193 reactive Cys were found in 79 unique proteins responding differentially in dormant, non‐dormant, abscisic, or gibberellic acid‐treated seed protein extracts from RL4137, a wheat cultivar with extreme dormancy. The identified proteins included groups that are redox‐, stress‐, and pathogen‐responsive, involved in protein synthesis and storage, are enzymes of carbohydrate metabolism, proteases, and those involved in transport and signal transduction. Two types of redox response could be detected: (i) a dramatic increase in protein thiol redox state in seeds during imbibition and hormonal treatment; (ii) higher antioxidant capacity related to sensing of a thr

Who reads Redox-sensitive Proteome and Antioxidant Strategies in Wheat Seed Dormancy Control?

Author

Natalia V. Bykova; Brenda Hoehn; Christof Rampitsch; Travis Banks; Jo-Ann Stebbing; Tao Fan; Ron Knox

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)