Can I read Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody : Role in human renal glomerular binding on EtoBox?

Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody : Role in human renal glomerular binding by Davin Chark; Anita Nutikka; Natasha Trusevych; Julia Kuzmina; Clifford Lingwood is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody : Role in human renal glomerular binding about?

The role of renal expression of the glycosphingolipid verotoxin receptor, globotriaosylceramide, in susceptibility to verotoxin‐induced hemolytic uremic syndrome is unclear. We show that a single glycosphingolipid can discriminate multiple specific ligands. Antibody detection of globotriaosylceramide in renal sections does not necessarily predict verotoxin binding. The deoxyglobotriaosylceramide binding profile for verotoxin 1, verotoxin 2 and monoclonal anti‐globotriaosylceramide are distinct. Anti‐globotriaosylceramide had greater dependency on the intact α‐galactose and reducing glucose of globotriaosylceramide than verotoxin 1, while verotoxin 2 was intermediate. These ligands differentially stained human kidney sections. Glomerulopathy is the primary verotoxin‐associated pathology in hemolytic uremic syndrome. For most samples, verotoxin 1 immunostaining within adult glomeruli was observed (type A). Some samples, however, lacked glomerular binding (type B). Anti‐globotriaosylceramide (and less effectively, verotoxin 2) stained all glomeruli. Verotoxin 1/anti‐globotriaosylceramide tubular staining was comparable. Type B glomerular/tubular globotriaosylceramide showed minor, but

Who reads Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody : Role in human renal glomerular binding?

Author

Davin Chark; Anita Nutikka; Natasha Trusevych; Julia Kuzmina; Clifford Lingwood

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)