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Mass spectrometry assisted assignments of binding and cleavage sites of copper(II) and platinum(II) complexes towards oxidized insulin B chain by Jin Hong; Ren Miao; Chunmei Zhao; Jiang Jiang; Huiwei Tang; Zijian Guo; Longgen Zhu is a Chemistry article available to read on EtoBox.

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## Abstract Interaction of __cis__‐[Pt(en)(H~2~O)~2~]^2+^ and [CuL(H~2~O)]^2+^, where L is 2‐[bis(2‐aminoethyl)amino]ethanol, with oxidized insulin B chain in molar ratio of 1 : 1, 1 : 2 and 1 : 3 at pH 2.5 and 40 °C has been investigated by electrospray ionization mass spectrometry (ESI‐MS) and tandem mass spectrometry (MS/MS). The results show that the binding sites of the two complexes with oxidized insulin B chain are terminal NH~2~, imidazole groups of His5 and His10. The hydrolytic cleavage studies show that the [CuL(H~2~O)]^2+^, upon a pendant hydroxyl group of the ligand, selectively cleaves the peptide bonds at Gly8–Ser9, Asn3–Gln4 and Phe1–Val2, and the __cis__‐[Pt(en)(H~2~O)~2~]^2+^ only cleaves the peptide bond at His10–Leu11. This is the first report of __cis__‐[Pt(en)(H~2~O)~2~]^2+^‐promoted cleavage of His–X peptide bond. Copyright © 2006 John Wiley & Sons, Ltd.

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Author

Jin Hong; Ren Miao; Chunmei Zhao; Jiang Jiang; Huiwei Tang; Zijian Guo; Longgen Zhu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)