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Sediment prokaryotic assembly, methane cycling, and ammonia oxidation potentials in response to increasing antibiotic pollu ... 1 1 Introduction 2 2 Materials and methods 2 2.1 Sample collection 2 2.2 Measurements of sediment biogeochemical properties 2 2.3 DNA extraction, microbial 16S rDNA amplification, and Illumina sequencing 2 2.4 Illumina data processing 3 2.5 Statistical analysis 3 2.6 Ecological network construction 3 3 Results 3 3.1 Variations in geochemical factors and enzyme activities among sites 3 3.2 Spatial distribution of sediment bacterial and archaeal communities 3 3.3 Interplay among prokaryotic communities, geochemical variables, antibiotics, and enzyme activities 4 3.4 Nonlinear response of bacteria and archaea to increasing antibiotic pollution 4 3.5 Screening indicator taxa for diagnosing antibiotic concentration 4 3.6 Identification of taxa involved in methane cycling 6 3.7 Ammonia oxidation process in response to increasing antibiotic pollution 6 4 Discussions 6 5 Conclusions 10 CRediT authorship contribution statement 10 Declaration of Competing Interest 10 Acknowledgements 10 Appendix A Supporting information 10 References 10

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Language

EN

Category

nonfiction