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Identification and Characterization of Liver Nonparenchymal Cells by Flow Cytometry by Mark Doolittle; Roger Bohman; Andres Durstenfeld; Joseph Cascarano is a Medicine article available to read on EtoBox.

What is Identification and Characterization of Liver Nonparenchymal Cells by Flow Cytometry about?

Our objective was to correlate biochemical or functional properties that could be measured by fluorescence to individual nonparenchymal cell types by the simultaneous detection of forward angle light scatter and fluorescence. Cells were released from liver following selective digestion of hepatocytes by Pronase perfusion. To fractionate the cells according to size, isolates were subjected to unit gravity sedimentation on a 1 to 5% sucrose gradient. Flow cytometry was used to analyze the resulting fractions as well as the original unfractionated cells. This reaffirmed that forward angle light scatter is linearly related to the sedimentation velocity (size) of the cells and that it can be used to assess cell sizes in an unfractionated population. Endocytosis of fluorescently tagged particulates was studied by injecting either fluorescein isothiocyanate-tagged colloidal albumin or Coumarin-tagged latex beads (0.57 micron diameter) prior to Pronase perfusion. Beads were found only in the largest cells (Kupffer cells). Since endothelial cells could endocytose only colloidal albumin, they were readily differentiated from nonendocytosing, nonsinusoidal cells ("lymphocytes"). Staining cell

Who reads Identification and Characterization of Liver Nonparenchymal Cells by Flow Cytometry?

Author

Mark Doolittle; Roger Bohman; Andres Durstenfeld; Joseph Cascarano

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Elsevier BV; Wiley; Research Square (ISSN 0270-9139)

Published

1987

Language

EN

Field

Medicine (Health Sciences)