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Analysis of Altered Gene Expression in Rat Soleus Muscle Atrophied by Disuse by Nathalie Cros; Andrei V. Tkatchenko; Didier F. Pisani; Lilian Leclerc; Jean J. Léger; Jean-François Marini; Claude A. Dechesne is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analysis of Altered Gene Expression in Rat Soleus Muscle Atrophied by Disuse about?
## Abstract The present study involved a global analysis of genes whose expression was modified in rat soleus muscle atrophied after hindlimb suspension (HS). HS muscle unloading is a common model for muscle disuse that especially affects antigravity slow‐twitch muscles such as the soleus muscle. A cDNA cloning strategy, based on suppression subtractive hybridization technology, led to the construction of two normalized soleus muscle cDNA libraries that were subtracted in opposite directions, i.e., atrophied soleus muscle cDNAs subtracted by control cDNAs and vice versa. Differential screening of the two libraries revealed 34 genes with altered expression in HS soleus muscle, including 11 novel cDNAs, in addition to the 2X and 2B myosin heavy chain genes expressed only in soleus muscles after HS. Gene up‐ and down‐regulations were quantified by reverse Northern blot and classical Northern blot analysis. The 25 genes with known functions fell into seven important functional categories. The homogeneity of gene alterations within each category gave several clues for unraveling the interplay of cellular events implied in the muscle atrophy phenotype. In particular, our results indicate
Who reads Analysis of Altered Gene Expression in Rat Soleus Muscle Atrophied by Disuse?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nathalie Cros; Andrei V. Tkatchenko; Didier F. Pisani; Lilian Leclerc; Jean J. Léger; Jean-François Marini; Claude A. Dechesne
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)