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Can I read Human interleukin-2 production in insect (Trichoplusia ni) larvae: Effects and partial control of proteolysis on EtoBox?
Human interleukin-2 production in insect (Trichoplusia ni) larvae: Effects and partial control of proteolysis by Minh-Quan Pham; Susanna Naggie; Marjorie Wier; Hyung Joon Cha; William E. Bentley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Human interleukin-2 production in insect (Trichoplusia ni) larvae: Effects and partial control of proteolysis about?
Many eukaryotic proteins have been successfully expressed in insect cells infected with a baculovirus in which the foreign gene has been placed under the control of a viral promoter. This system can be costly at large scale due to the quality of virus stock, problems of oxygen transfer, and severity of large-scale contamination. To circumvent this problem, we have investigated the expression of a foreign protein, human interleukin-2 (IL-2), in insect larvae, Trichoplusia ni, infected with the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV). The IL-2 gene was placed under control of the p10 promoter so that the polyhedra remained intact for efficient primary infection. From our results, it was clear that early infection limited larval growth and late infection delayed product production until near pupation, hence infection timing was important. Also, the harvest time was crucial for obtaining high yield, because IL-2 production had a sharp optimal peak with a time of occurrence dependent on both temperature and the initial amount of infection virus. Specifically, we found that, by raising the infection temperature to 30°C, we more than doubled the protein produ
Who reads Human interleukin-2 production in insect (Trichoplusia ni) larvae: Effects and partial control of proteolysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Minh-Quan Pham; Susanna Naggie; Marjorie Wier; Hyung Joon Cha; William E. Bentley
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)