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Supercoiled DNA Migration in Gel Electrophoresis by Tati Machado is a document available to read on EtoBox.
This lab report describes an experiment analyzing the topological forms of supercoiled plasmid DNA using agarose gel electrophoresis. Plasmid DNA was isolated from bacterial cells and treated with the intercalating agent chloroquine, which changes the supercoiling state. The DNA samples were then separated on an agarose gel based on their linking number. The gel showed bands corresponding to different topological isomers ranging from fully negatively supercoiled to fully relaxed. However, the full experimen
- Author
- Tati Machado
- Language
- EN