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Determination and Application of Empirically Derived Detergent Phase Boundaries to Effectively Crystallize Membrane Proteins by Mary Koszelak-Rosenblum; Adam Krol; Namrita Mozumdar; Kristin Wunsch; Adam Ferin; Eleanor Cook; Christina K. Veatch; Raymond Nagel; Joseph R. Luft; George T. DeTitta; Michael G. Malkowski is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Determination and Application of Empirically Derived Detergent Phase Boundaries to Effectively Crystallize Membrane Proteins about?
## Abstract Elucidating the structures of membrane proteins is essential to our understanding of disease states and a critical component in the rational design of drugs. Structural characterization of a membrane protein begins with its detergent solubilization from the lipid bilayer and its purification within a functionally stable protein‐detergent complex (PDC). Crystallization of the PDC typically occurs by changing the solution environment to decrease solubility and promote interactions between exposed hydrophilic surface residues. As membrane proteins have been observed to form crystals close to the phase separation boundaries of the detergent used to form the PDC, knowledge of these boundaries under different chemical conditions provides a foundation to rationally design crystallization screens. We have carried out dye‐based detergent phase partitioning studies using different combinations of 10 polyethylene glycols (PEG), 11 salts, and 11 detergents to generate a significant amount of chemically diverse phase boundary data. The resulting curves were used to guide the formulation of a 1536‐cocktail crystallization screen for membrane proteins. We are making both the experimen
Who reads Determination and Application of Empirically Derived Detergent Phase Boundaries to Effectively Crystallize Membrane Proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mary Koszelak-Rosenblum; Adam Krol; Namrita Mozumdar; Kristin Wunsch; Adam Ferin; Eleanor Cook; Christina K. Veatch; Raymond Nagel; Joseph R. Luft; George T. DeTitta; Michael G. Malkowski
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)