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Can I read l-Proline improves the cytoplasmic maturation of mouse oocyte by regulating glutathione-related redox homeostasis on EtoBox?
l-Proline improves the cytoplasmic maturation of mouse oocyte by regulating glutathione-related redox homeostasis by Ning Liu; Xuemeng Si; Yun Ji; Qing Yang; Jun Bai; Yu He; Hai Jia; Zhuan Song; Jingqing Chen; Ling Yang; Shenming Zeng; Ying Yang; Zhenlong Wu is a Medicine article available to read on EtoBox.
What is l-Proline improves the cytoplasmic maturation of mouse oocyte by regulating glutathione-related redox homeostasis about?
Recent studies have shown that l-proline (proline) is an antioxidant to protect cells from oxidative stress in vivo and in vitro. Glutathione (GSH) is a major cellular redox regulator involved in controlling redox balance and is regarded as one of the key indices to predict the cytoplasmic maturation of oocytes. The objectives of this study are to investigate the effect of proline on the developmental potential of mouse oocytes and to determine the role of gap junctional communication (GJC) on intraoocyte GSH concentration during in vitro maturation (IVM). Compared with control (0 mmol/L), 0.5 mmol/L proline supplementation enhanced rates of activated oocytes, 2-cell and 4-cell embryos, and blastocysts. Furthermore, 0.25 and 0.5 mmol/L proline supplementation markedly upregulated mRNA expression of glutamate-cysteine ligase catalytic subunit (GCLC) and glutamate-cysteine ligase modifier subunit (GCLM) in oocytes and cumulus cells, enhanced GSH concentration in oocytes, and reduced reactive oxygen species (ROS) level in oocytes. Interestingly, carbenoxolone disodium salt (CBX) treatment reduced GSH concentration in oocytes and the rate of early embryo development without proline inc
Who reads l-Proline improves the cytoplasmic maturation of mouse oocyte by regulating glutathione-related redox homeostasis?
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- Author
- Ning Liu; Xuemeng Si; Yun Ji; Qing Yang; Jun Bai; Yu He; Hai Jia; Zhuan Song; Jingqing Chen; Ling Yang; Shenming Zeng; Ying Yang; Zhenlong Wu
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Medicine (Health Sciences)