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This document provides instructions for an in-lab assignment on polyacrylamide gel electrophoresis. Students are asked to: 1) Calculate the molar mass of tryptophan and GFP using bioinformatics tools. 2) Determine the optimal pH for Coomassie blue staining based on its functional groups. 3) Explain how SDS ensures proteins migrate by mass rather than other properties. 4) Analyze provided SDS-PAGE gels by estimating protein band sizes, concentrations, and amino acid counts.

Author

Thysian

Language

EN