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Improved TLC systems for rapid resolution of phenyl thiohydantoin amino acids by R. Bhushan; V. K. Mahesh; P. V. Mallikharjun is a Chemistry article available to read on EtoBox.

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Two new solvent systems, n-hexane + propionic acid (26: 5, v/v) and chloroform + acetone (29 : 3, v/v), for the rapid resolution and identification of an 18-component mixture of phenylthiohydantoin amino acids are reported. Using these systems certain difficult combinations of phenylthiohydantoin amino acids are resolved. Two more solvent systems, viz chloroform + acetic acid (27 : 3, v/v) and chloroform + methanol (30 : 4, v/v), are developed to resolve phenylthiodantoin derivatives of aspartic and glutamic acids. Detection of PTH-amino acids. After development, the chromatograms were removed from the chambers. These were dried and exposed to iodine vapours to locate the PTH-amino acid as light yellow-brown spots on the chromatograms.

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Author

R. Bhushan; V. K. Mahesh; P. V. Mallikharjun

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0269-3879)

Published

1989

Language

EN

Field

Chemistry (Physical Sciences)