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Can I read Chemical synthesis, characterization and activity of RK-1, a novel α-defensin-related peptide on EtoBox?
Chemical synthesis, characterization and activity of RK-1, a novel α-defensin-related peptide by Nicola F. Dawson; David J. Craik; Ailsa M. Mcmanus; Stuart G. Dashper; Eric C. Reynolds; Geoffrey W. Tregear; Laszlo Otvos Jr; John D. Wade is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chemical synthesis, characterization and activity of RK-1, a novel α-defensin-related peptide about?
The 32-residue peptide, RK-1, a novel kidney-derived three disulfide-bonded member of the antimicrobial h-defensin family, was synthesized by the continuous flow Fmoc-solid phase method. The crude, cleaved and S-reduced linear peptide was both efficiently folded and oxidized in an acidic solution of aqueous dimethyl sulfoxide. Following purification of the resulting product, it was shown by a variety of analytical techniques, including matrix assisted laser desorption time of flight mass spectrometry, to possess a very high degree of purity. The disulfide bond pairing of the synthetic peptide was determined by 1 H-NMR spectroscopy and confirmed to be a Cys 1 -Cys 6 , Cys 2 -Cys 4 , Cys 3 -Cys 5 arrangement similar to other mammalian h-defensin peptides. The synthetic RK-1 was also shown to inhibit the growth of Escherichia coli type strain NCTC 10418.
Who reads Chemical synthesis, characterization and activity of RK-1, a novel α-defensin-related peptide?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nicola F. Dawson; David J. Craik; Ailsa M. Mcmanus; Stuart G. Dashper; Eric C. Reynolds; Geoffrey W. Tregear; Laszlo Otvos Jr; John D. Wade
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1075-2617)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)