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Measuring oxidative DNA damage and DNA repair using the yeast comet assay by Flávio Azevedo; Filipe Marques; Hanna Fokt; Rui Oliveira; Björn Johansson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the extent of DNA damage. This technique has been used for a variety of applications with several organisms, but only a few studies have been reported for __Saccharomyces cerevisiae__. A possible reason for this absence is that low cellular DNA content could hamper visualization. Here we report an optimization of the comet assay protocol for yeast cells that is robust and sensitive enough to reproducibly detect background DNA damage and oxidative damage caused by hydrogen peroxide. DNA repair was observed and quantified as diminishing comet tail length with time after oxidative stress removal in a process well described by first‐order kinetics with a tail length half‐life of 11 min at 37 °C. This is, to our knowledge, the first quantitative measurement of DNA repair kin

Who reads Measuring oxidative DNA damage and DNA repair using the yeast comet assay?

Author

Flávio Azevedo; Filipe Marques; Hanna Fokt; Rui Oliveira; Björn Johansson

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)