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Significant Antitumor Activity of Oncolytic Adenovirus Expressing Human Interferon-β for Hepatocellular Carcinoma by Ling Feng He; Jin Fa Gu; Wen Hao Tang; Jun Kai Fan; Na Wei; Wei Guo Zou; Yan Hong Zhang; Li Li Zhao; Xin Yuan Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Significant Antitumor Activity of Oncolytic Adenovirus Expressing Human Interferon-β for Hepatocellular Carcinoma about?
## Abstract ## Background Human interferon‐β (IFN‐β) has been widely used in gene therapy for its antitumor activity but its therapeutic effect is limited. The conditionally replicative adenovirus ONYX‐015 (a E1B‐55‐kDa‐deleted adenovirus) targets well to tumor cells, but is not potent enough to cause significant tumor regression. To solve these problems, a tumor‐selective replicating adenovirus expressing IFN‐β was constructed in this study. ## Methods The oncolytic adenoviruses were generated by homologous recombination in packaging cells. The expression of the IFN‐β protein was detected by enzyme‐linked immunosorbent assay (ELISA). The antitumor efficacy of ZD55‐IFN‐β was evaluated in cell lines and human hepatocellular carcinoma xenografts in nude mice. ## Results ZD55‐IFN‐β can express much more IFN‐β than Ad‐IFN‐β because of the replication of the ZD55 vector. Our data showed that ZD55‐IFN‐β could exert a strong cytopathic effect on tumor cells (about 100‐fold higher than Ad‐IFN‐β or ONYX‐015). Moreover, no obvious cytotoxic or apoptotic effects were detected in normal cells infected with ZD55‐IFN‐β. ## Conclusions The antitumor efficacy of IFN‐β could be significantly improv
Who reads Significant Antitumor Activity of Oncolytic Adenovirus Expressing Human Interferon-β for Hepatocellular Carcinoma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ling Feng He; Jin Fa Gu; Wen Hao Tang; Jun Kai Fan; Na Wei; Wei Guo Zou; Yan Hong Zhang; Li Li Zhao; Xin Yuan Liu
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)