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Detection of PLGA-based nanoparticles at a single-cell level by synchrotron radiation FTIR spectromicroscopy and correlation with X-ray fluorescence microscopy by Giovanni Maria Severini; Lorella Pascolo; Barbara Bortot; Nuria Benseny-Cases; Alessandra Gianoncelli; Giovanni Tosi; Barbara Ruozi; Clara Rizzardi; Eleonora De Martino; Maria Angela Vandelli is a Materials Science article available to read on EtoBox.
What is Detection of PLGA-based nanoparticles at a single-cell level by synchrotron radiation FTIR spectromicroscopy and correlation with X-ray fluorescence microscopy about?
Poly-lactide-co-glycolide (PLGA) is one of the few polymers approved by the US Food and Drug Administration as a carrier for drug administration in humans; therefore, it is one of the most used materials in the formulation of polymeric nanoparticles (NPs) for therapeutic purposes. Because the cellular uptake of polymeric NPs is a hot topic in the nanomedicine field, the development of techniques able to ensure incontrovertible evidence of the presence of NPs in the cells plays a key role in gaining understanding of their therapeutic potential. On the strength of this premise, this article aims to evaluate the application of synchrotron radiation-based Fourier transform infrared spectroscopy (SR-FTIR) spectromicroscopy and SR X-ray fluorescence (SR-XRF) microscopy in the study of the in vitro interaction of PLGA NPs with cells. To reach this goal, we used PLGA NPs, sized around 200 nm and loaded with superparamagnetic iron oxide NPs (PLGA-IO-NPs; Fe~3~O~4~; size, 10–15 nm). After exposing human mesothelial (MeT5A) cells to PLGA-IO-NPs (0.1 mg/mL), the cells were analyzed after fixation both by SR-FTIR spectromicroscopy and SR-XRF microscopy setups. SR-FTIR-SM enabled the detection o
Who reads Detection of PLGA-based nanoparticles at a single-cell level by synchrotron radiation FTIR spectromicroscopy and correlation with X-ray fluorescence microscopy?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Giovanni Maria Severini; Lorella Pascolo; Barbara Bortot; Nuria Benseny-Cases; Alessandra Gianoncelli; Giovanni Tosi; Barbara Ruozi; Clara Rizzardi; Eleonora De Martino; Maria Angela Vandelli
- Publisher
- Dove Press Ltd.; Dove Medical Press; Dove Medical Press Ltd; [Auckland N.Z.]: Dovepress; Informa UK Limited (ISSN 1176-9114)
- Published
- 2014
- Language
- EN
- Field
- Materials Science (Physical Sciences)