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Genome organization in the human sperm nucleus studied by FISH and confocal microscopy by M. Hazzouri; S. Rousseaux; F. Mongelard; Y. Usson; R. Pelletier; A.K. Faure; C. Vourc'h; B. Sèle is a Medicine article available to read on EtoBox.

What is Genome organization in the human sperm nucleus studied by FISH and confocal microscopy about?

The sperm nucleus has a unique chromatin structure where the DNA is highly condensed and associated with specific proteins, the protamines. It is a nondividing cell which is also transcriptionally inactive. After fusion with an oocyte, the sperm nucleus undergoes decondensation and, in the same time, starts replication and transcription. It has been suggested that somatic chromosomes during interphase are organized in territories which display a cell type and cell cycle specific distribution. The purpose of this work was to investigate whether chromosomes would also have a specific distribution in the sperm nucleus, which could be related to its inactive state, and have implications on the early stages of fertilization. In the present study, centromeric and telomeric sequences were detected by fluorescent techniques performed on human decondensed spermatozoa. Chromosome painting probes were used to detect the chromosome X and chromosome 13 on interphase sperm nuclei. The fluorescent signals were captured in 3D with a confocal microscope. For each of these chromatin structures, the volume, position, and distribution of the signals were analyzed in samples of 30 nuclei with the help

Who reads Genome organization in the human sperm nucleus studied by FISH and confocal microscopy?

Author

M. Hazzouri; S. Rousseaux; F. Mongelard; Y. Usson; R. Pelletier; A.K. Faure; C. Vourc'h; B. Sèle

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)

Published

2000

Language

EN

Field

Medicine (Life Sciences)