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Fungal Responsive Fatty Acid Acetylenases Occur Widely in Evolutionarily Distant Plant Families by Edgar B. Cahoon; Judy A. Schnurr; Errol A. Huffman; Robert E. Minto is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary The fungal elicitor‐induced __ELI12__ gene from parsley has been previously shown to encode a divergent form of the Δ^12^‐oleic acid desaturase. In this report, we show that the __ELI12__ gene product is a fatty acid acetylenase or a triple‐bond‐forming enzyme. Expression of this enzyme in transgenic soybean seeds was accompanied by the accumulation of the Δ^12^‐acetylenic fatty acids, crepenynic and dehydrocrepenynic acids. Using PCR with degenerate oligonucleotides, we also show that homologs of the __ELI12__ gene are present in other members of the Apiaceae family. In addition, cDNAs for divergent forms of the Δ^12^‐oleic acid desaturase were detected among the expressed sequence tags (ESTs) from English ivy, an Araliaceae species, and sunflower, an Asteraceae species. As with the __ELI12__ gene, expression of these cDNAs in transgenic soybean embryos was accompanied by the accumulation of crepenynic and dehydrocrepenynic acids. Homologs of the sunflower acetylenase gene were also detected in other Asteraceae species, as revealed by PCR analysis of isolated genomic DNA. Results from Northern blot and EST analyses indicated that the expression of the sunflower gene, li

Who reads Fungal Responsive Fatty Acid Acetylenases Occur Widely in Evolutionarily Distant Plant Families?

Author

Edgar B. Cahoon; Judy A. Schnurr; Errol A. Huffman; Robert E. Minto

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)