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Extracellular Calcium Deprivation in Astrocytes: Regulation of mRNA Expression and Apoptosis by Roberto Chiesa; Nadia Angeretti; Roberto Del Bo; Elisa Lucca; Elettra Munna; Gianluigi Forloni is a Neuroscience article available to read on EtoBox.
What is Extracellular Calcium Deprivation in Astrocytes: Regulation of mRNA Expression and Apoptosis about?
Abstract: Cell viability and gene expression were studied in primary astroglial cells cultured in a nominally calcium‐free medium. Ca^2+^ deprivation reduced progressively the astrocytes' viability, starting from 12 h; the restoration of a normal Ca^2+^ concentration (1.8 m__M__) in the medium after 12‐h deprivation reversed the degenerative effect within 24 h. Biochemical and morphological examinations indicated that cell death induced by Ca^2+^ deprivation was mediated by apoptosis. This was associated with the expression of c‐__fos__, c‐__jun__, and c‐__myc__, which, with different time courses, were induced in astrocytes after Ca^2+^ deprivation. Furthermore, shifting to a Ca^2+^‐free medium modified the expression of __Ich‐1__~S~ transcript and rapidly increased intracellular cyclic AMP, which has been implicated in the transcriptional activation of immediate‐early genes. The absence of Ca^2+^ in the medium reduced the expression of constitutive proteins such as α‐actin, clusterin, glial fibrillary acidic protein, amyloid precursor protein, and glucose‐6‐phosphate dehydrogenase. The expression of these mRNAs was reduced >50% after 8 h of Ca^2+^ deprivation, when the effect on
Who reads Extracellular Calcium Deprivation in Astrocytes: Regulation of mRNA Expression and Apoptosis?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Roberto Chiesa; Nadia Angeretti; Roberto Del Bo; Elisa Lucca; Elettra Munna; Gianluigi Forloni
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1998
- Language
- EN
- Field
- Neuroscience (Life Sciences)