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Inhibition of oxidative stress produced by plasma membrane NADH oxidase delays low-potassium-induced apoptosis of cerebellar granule cells by Francisco Javier Martín-Romero; Elena García-Martín; Carlos Gutiérrez-Merino is a Neuroscience article available to read on EtoBox.

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## Abstract From 1 to 3 h after the onset of cerebellar granule cells (CGC) apoptosis in a low‐K^+^(5 mm KCl) medium there was a large decay of NADH and a 2.5‐fold increase of the rate of reactive oxygen species (ROS) production (measured using CGC loaded with dichlorodihydrofluorescein). During the same time period, the ascorbate‐dependent NADH oxidase activity, which accounted for more than 90% of both total NADH oxidase activity and NADH‐dependent ^·^O~2~^–^ production of CGC lysates, increased 2.5‐ to threefold. The stimulation of the ascorbate‐dependent NADH oxidase activity by oxidized cytochrome c, 2.5‐fold at saturation with a K~0.5~ of 4–5 μm cytochrome c, can at least partially explain this activation. The plasma membrane ascorbate‐dependent NADH oxidase activity accounted for more than 70% of the total activity (both in terms of NADH oxidase and ^·^O~2~^–^ release) of CGC lysates. 4‐Hydroxyquinazoline (4‐HQ), which was found to block this apoptotic process, prevented the increase of ROS production. 4‐HQ protection against cell viability loss and DNA fragmentation correlated with the inhibition by 4‐HQ of the ascorbate‐dependent NADH oxidase activity of CGC lysates, showi

Who reads Inhibition of oxidative stress produced by plasma membrane NADH oxidase delays low-potassium-induced apoptosis of cerebellar granule cells?

Author

Francisco Javier Martín-Romero; Elena García-Martín; Carlos Gutiérrez-Merino

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)