Can I read Preparation of graphene oxide quantum dots from waste toner, and their application to a fluorometric DNA hybridization assay on EtoBox?

Preparation of graphene oxide quantum dots from waste toner, and their application to a fluorometric DNA hybridization assay by Qiang Xu; Yan Gong; Zhifeng Zhang; Yanming Miao; Dongxia Li; Guiqin Yan is a Chemistry article available to read on EtoBox.

What is Preparation of graphene oxide quantum dots from waste toner, and their application to a fluorometric DNA hybridization assay about?

A one-pot hydrothermal method was developed for the synthesis of graphene oxide quantum dots (GOQDs). It is making use of toner waste as the precursor and HO as the oxidant. Synthesis takes 4 h and does not require strong acids or complex purification steps and does not produce environmentally harmful metal ions. The GOQDs display blue fluorescence with excitation/emission maxima at 340/445 nm. The feasibility of detecting specific DNA sequence was promoted using polyethyleneimine to modify the GOQDs surface. A method was developed to recognized a specific DNA sequence. This is based on electrostatic aggregation of GOQDs and ssDNA labeled with Dabcyl at the 3' end, which promotes fluorescence quenching of GOQDs. The possible fluorescence quenching mechanism (which is mainly dynamic) was investigated using the Stern-Volmer equation. When a target sequence was added, which is complementary to the ssDNA, the dabcyl-labeled ssDNA is released due to strict complementary base pairing. This promotes fluorescence recovery of GOQDs. The assay has a 0.17 nM detection limit and a linear range of 0.5-30 nM. The method was used to quantify specific DNA sequences from extracts of genetically mod

Who reads Preparation of graphene oxide quantum dots from waste toner, and their application to a fluorometric DNA hybridization assay?

Author

Qiang Xu; Yan Gong; Zhifeng Zhang; Yanming Miao; Dongxia Li; Guiqin Yan

Publisher

Springer Science and Business Media LLC

Published

2019

Language

EN

Field

Chemistry (Physical Sciences)