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A sensitive “turn-on” fluorescent biosensor for the detection of biomarkers based on three-dimensional DNA walker by Yunyan Xie; Shihui Si; Chunyan Deng; Huiyun Wu is a Chemistry article available to read on EtoBox.

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Three-dimensional (3D) DNA walker have been widely acknowledged as a promising strategy for improving the detection sensitivity of biosensors. In this work, a sensitive “turn-on” fluorescent biosensor for the detection of biomarkers based on three-dimensional DNA walker was developed. Herein, DNA walker strands, fluorophores (FAM and Cy5)-labeled hairpin substrate strands, HER2-specific aptamer, and gold nanoparticles (AuNPs) were involved to fabricate the nanoprobe1 (nanop1) for human epidermal growth factor receptor 2 (HER2) and nanoprobe2 (nanop2) for microRNA-21 (miRNA-21). The composite fluorescent probe comprising of nanop1 and nanop2 was employed for the multiplex determination of HER2 and miRNA-21 without cross interference. This fluorescent biosensor was sensitive with the detection limits of 0.01 ng mL−1 for HER2 and 0.018 pM for miRNA-21, which was mainly due to the signal amplification of Nb.BbvcI powered 3D DNA walker. Moreover, this biosensor was selective and reliable for the detection of HER2 and miRNA-21 spiked in the serum samples. Therefore, this present 3D DNA walker-based biosensor is not only highly sensitive and specific, but also is accurate because of the m

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Author

Yunyan Xie; Shihui Si; Chunyan Deng; Huiyun Wu

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Chemistry (Physical Sciences)