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Evaluation of Zona Pellucida Birefringence Intensity During in Vitro Maturation of Oocytes from Stimulated Cycles by Claudia G Petersen; Laura D Vagnini; Ana L Mauri; Fabiana C Massaro; Liliane FI Silva; Mario Cavagna; Ricardo LR Baruffi; Joao BA Oliveira; José G Franco is a Medicine article available to read on EtoBox.

What is Evaluation of Zona Pellucida Birefringence Intensity During in Vitro Maturation of Oocytes from Stimulated Cycles about?

## Background This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles. ## Results The ZP-BF was evaluated under an inverted microscope with a polarizing optical system and was scored as high/positive (when the ZP image presented a uniform and intense birefringence) or low/negative (when the image presented moderate and heterogeneous birefringence). CM was analyzed by evaluating the distribution of cortical granules (CGs) throughout the ooplasm by immunofluorescence staining. CM was classified as: complete, when CG was localized in the periphery; incomplete, when oocytes presented a cluster of CGs in the center; or in transition, when oocytes had both in clusters throughout cytoplasm and distributed in a layer in the cytoplasm periphery Nuclear maturation: From a total of 83 germinal vesicle (GV) stage oocytes, 58 of oocytes (69.9%) reached NM at the metaphase II stage. From these 58 oocytes matured in vitro, the high/positively scoring ZP-BF was presented in 82.7% of oocytes at the GV stage, in 75.8% of oocytes when at the met

Who reads Evaluation of Zona Pellucida Birefringence Intensity During in Vitro Maturation of Oocytes from Stimulated Cycles?

Author

Claudia G Petersen; Laura D Vagnini; Ana L Mauri; Fabiana C Massaro; Liliane FI Silva; Mario Cavagna; Ricardo LR Baruffi; Joao BA Oliveira; José G Franco

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2003-; Springer Science and Business Media LLC (ISSN 1477-7827)

Published

2011

Language

EN

Field

Medicine (Health Sciences)