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Disposable DNA electrochemical sensor for hybridization detection1This paper was presented at the Fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1 by G. Marrazza; I. Chianella; M. Mascini is a Engineering article available to read on EtoBox.

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A disposable electrochemical sensor for the detection of short DNA sequences is described. Synthetic single-stranded oligonucleotides have been immobilized onto graphite screen printed electrodes with two procedures, the first involving the binding of avidinbiotinylated oligonucleotide and the second adsorption at a controlled potential. The probes were hybridized with different concentrations of complementary sequences. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry and chronopotentiometric stripping analysis using daunomycin hydrochloride as indicator of hybridization reaction. The probe immobilization step, the hybridization event and the indicator detection, have been optimized. The DNA sensor obtained by adsorption at a controlled potential was able to detect 1 microgram/ml of target sequence in the buffer solution using chronopotentiometric stripping analysis.

Who reads Disposable DNA electrochemical sensor for hybridization detection1This paper was presented at the Fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1?

Author

G. Marrazza; I. Chianella; M. Mascini

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

1999

Language

EN

Field

Engineering (Physical Sciences)