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Dexamethasone inhibits maturation, cytokine production and FcεRI expression of human cord blood-derived mast cells by S. J. Smith; A. M. Piliponsky; F. Rosenhead; U. Elchalal; A. Nagler; F. Levi-Schaffer is a Medicine article available to read on EtoBox.
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## Summary **Background** Mast cells are responsible for eliciting the early phase and for contributing to the development of the late phase of allergic reactions, through the release of cytokines and other inflammatory mediators. **Objective** To assess whether the glucocorticoid dexamethasone has a direct effect on mast cell progenitor maturation and on mature cord blood‐derived mast cell properties. **Methods** Mast cells were obtained by culturing human umbilical cord blood mononuclear cells with stem cell factor, IL‐6 and prostaglandin E~2~. Mast cell numbers were assessed by Toluidine Blue staining and immunocytochemistry of tryptase positive cells. The expression of FcεRI, CD49d and c‐kit was assessed by flow cytometry. Histamine release was determined by a radioenzymatic assay. Cys‐LT, GM‐CSF and TNF‐α production and release were determined by ELISA. **Results** Dexamethasone (10^−6^ M−10^−9^ M) time‐ and dose‐dependently inhibited the maturation of the mast cell progenitors. Dexamethasone did not affect the basal expression of FcεRI, CD49d and c‐kit, but it inhibited the IgE‐dependent enhanced expression of FcεRI. Dexamethasone (10^−6^ M−10^−9^ M) had no significant effect
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- Author
- S. J. Smith; A. M. Piliponsky; F. Rosenhead; U. Elchalal; A. Nagler; F. Levi-Schaffer
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0954-7894)
- Published
- 2002
- Language
- EN
- Field
- Medicine (Health Sciences)