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Compositional analysis of small peptides using capillary electrophoresis and Ru(bpy)33+-based chemiluminescence detection by Howard P Hendrickson; Paul Anderson; Xin Wang; Zidia Pittman; Donald R Bobbitt is a Chemistry article available to read on EtoBox.

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## Ž . 3q Ru bpy -based chemiluminescence detection of underivatized amino acids was coupled to capillary electrophore-3 sis and evaluated as a separation and detection methodology for the compositional analysis of peptides and proteins. Two tripeptides were studied: glycine᎐phenylalanine᎐alanine, and valine᎐proline᎐leucine, and the method was demonstrated to be quantitative and reproducible at the 10-g and 5-g peptide levels, with a limit of detection Ž . Ž . Ž . 3q LOD of 2.5 pmol for gly᎐phe᎐ala and 80 fmol for val᎐pro᎐leu SrNs 2 . Ru bpy -based chemiluminescence 3 detection coupled to CE offers a sensitive and time-efficient method for the determination of the amino acid composition of peptides or proteins without derivatization. The detection limits for most amino acids were demonstrated to be at, or below the level achievable using ninhydrin or phenyl isothiocyanate derivatization. Amino acids were identified by their migration time through the capillary and their relative luminescence. The relative Ž . 3q luminescent response obtained upon reaction of Ru bpy with amino acids was dependent upon the amino acid 3 R-group at the ␣-carbon, and the relative response may be useful

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Author

Howard P Hendrickson; Paul Anderson; Xin Wang; Zidia Pittman; Donald R Bobbitt

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0026-265X)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)