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Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne by Yang, Wen-Jia; Xu, Kang-Kang; Yan, Yi; Li, Can; Jin, Dao-Chao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne about?

Chitin deacetylases (CDAs) are chitin-modifying enzymes known to play vital roles in insect metamorphosis and development. In this study, we identified and characterized a __chitin deacetylase__ __1__ gene (__LsCDA1__) from the cigarette beetle __Lasioderma serricorne__. __LsCDA1__ contains a 1614 bp open reading frame encoding a protein of 537 amino acids that includes domain structures typical of CDAs. __LsCDA1__ was mainly expressed in the late larval and late pupal stages. In larval tissues, the highest level of __LsCDA1__ was detected in the integument. The expression of __LsCDA1__ was induced by 20-hydroxyecdysone (20E) in vivo, and it was significantly suppressed by knocking down the expression of ecdysteroidogenesis genes and 20E signaling genes. RNA interference (RNAi)-aided silencing of __LsCDA1__ in fifth-instar larvae prevented the larval–pupal molt and caused 75% larval mortality. In the late pupal stage, depletion of __LsCDA1__ resulted in the inhibition of pupal growth and wing abnormalities, and the expression levels of four wing development-related genes (__LsDY__, __LsWG__, __LsVG__, and __LsAP__) were dramatically decreased. Meanwhile, the chitin contents of __Ls

Who reads Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne?

Author

Yang, Wen-Jia; Xu, Kang-Kang; Yan, Yi; Li, Can; Jin, Dao-Chao

Publisher

Molecular Diversity Preservation International; MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: MDPI Center, 2000- (ISSN 1422-0067)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)